The Primacy of Moringa (Moringa oleiferaLam.) in Boosting Nutrition Statusand Immunity Defence Amidst the COVID-19 Catastrophe: A Perspective br

A severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) led novel coronavirus disease (COVID-19)outbreak spread through China has become the biggest global public health challenge today. The virus upon sev-eral mutations has led to the resurgence of more infectious and lethal variants infecting over 298 million peoplewith more than 5.46 million deaths worldwide by the end of December, 2021. Though vaccines are available, var-ious preventive measures particularly a high body immunity is still extremely important which determines thelikelihood of disease severity and subsequent recovery in the current and future pandemics. This review acknowl-edges the potentiality of miraculousMoringa oleiferaLam. against recently evolved novel coronavirus and accom-panying health complications. Moringa a well-proven super-food, densely packed with an abundant quantity of92 minerals, several vitamins, 46 antioxidants, and numerous bioactive compounds, thus own a massive thera-peutic potential for healing all levels of nutritional deficiencies and poor immunities and cure above 300 diseases.Moringa acts as anti-asthmatic, anti-cancerous, anti-diabetic, anti-inflammatory, hypotensive, hepatic, renal andcardio-protective, and anti-viral in nature. Thus it may reduce the severity of COVID-19 infections and associatedserious medical emergencies. In addition, self-isolation at home or the workplace has put people at increased riskof physical and mental sicknesses, which could be simply addressed by integrating this wonderful plant intoeveryday diet. Furthermore, the immune-modulatory properties and viral inhibiting nature of moringa contributeto reduced risk of COVID-19 infection and quicker recovery from its symptoms. As per the existing pieces of literature, it is a great time to harness the esteemed moringa for safeguarding people from the terrible ongoingCOVID-19 situation and other future pandemics

The effectiveness and limitations of digital images for taxonomic research

In response to the worldwide coronavirus outbreak, which effectively shut down fieldwork, laboratory and herbarium-based studies, an evaluation was made of the effectiveness and limitations of undertaking a virtual taxonomic study using only online herbarium specimen resources related to the genus Madhuca (Sapotaceae) for the Flora of Singapore. The study demonstrated the immense value of digital images to basic taxonomic research but also found that diagnostic micro-morphological characters, often critical in defining species boundaries, cannot be seen in many digital images, even at high resolution. Several recommendations are made on how to maximise the utility of online herbarium specimen images to help facilitate future taxonomic research, though it is clear that physical access to herbarium specimens remains essential.

Being a Farmer in Austria during COVID-19-A Qualitative Study on Challenges and Opportunities

To assess the effects of COVID-19 on Austrian farmers, a qualitative study design including computer-aided, qualitative content analysis was applied. Interviews with 34 Austrian farmers covering a broad spectrum of the sector were conducted to identify the impacts, obstacles, and opportunities during the first year of the COVID-19 pandemic. The results show that the diverse and heterogeneous impacts of COVID-19 on farms created significant challenges but also opportunities. Negative impacts included sales difficulties due to the closure of the hospitality industry and farmers markets, the ban on non-agricultural activities, the disrupted availability of workers and agricultural inputs, and the related additional workload. Mitigation of negative effects through political measures and interest groups showed limited effectiveness. Nevertheless, the functionality of the agricultural sector was maintained. During the initial phase of the pandemic, direct impacts on agricultural production were minimal and adjustments were adequate. There were also positive effects on parts of the agricultural sector, such as a boost in direct sales, increased demand in the food retail sector, and improved public perception of agriculture and farmers. Long-term changes in farms have been triggered and/or reinforced by the COVID-19 pandemic, mainly driven by digitization, investments in product and/or process innovations, and adjustments concerning marketing.

PLANTS SECONDARY METABOLITES (PSMS), AS AN INVESTIGATIONAL SOURCE AGAINST COVID-19 FROM FLORA OF PAKISTAN

Plant Secondary Metabolites (PSMs) are naturally occurring organic compounds inside the plant produced in response to any internal or external environmental stress. These organic chemicals are in different forms (Terpenoids, polyphenols, alkaloids, etc.). PSMs are an active source of medicines against many types of viral as well as microbial diseases. Pakistani flora is also a rich source of medicinal plants, and their therapeutic range has great importance. These plants are already in use against various types of microbial diseases. The primary aim to write this paper is to highlight PSMs of medicinal plants of Pakistani flora, which can be effective against COVID-19.

LC-HRMS Based Approach for Identification and Quantification Analysis of Chemical Constituents of Sea Cucumbers from Aegean Sea - Their Cytotoxic and Antiviral Potentials

There are nearly 1200 species of sea cucumber in the world's seas. Among these creatures included in the Holothuroidea class, 37 species show distribution in the Mediterranean and Aegean Sea. The purpose of this study is to determine the chemical content and biological potent of five sea cucumber species, Holothuria tubulosa, Holothuria poli, Holothuria mammata, Holothuria sanctori and Stichopus regalis which were collected from the Aegean Sea. The detailed flavonoid, phenolic and triterpene contents were determined by LCHR/MS. Cytotoxic activities against several cancer cell lines, MDA-MB-231, PC-3, A549, PANC-1, HEPG2 and a healthy cell line CCD-34LU were performed by MTT method. Antiviral activities of the samples were measured as virucidal activity against avian coronavirus by in ovo. According to the results of LC-HRMS analysis, H. sanctori, H. poli and S. regalis had the richest chemical content diversity in terms of examined triterpene compounds. Fumaric acid was detected as the most abundant substance in all sea cucumber species. H. tubulosa had a highly toxic effect on all the tested cells. The best cytotoxic activity on A549 cells was seen in H. mammata, H. sanctori and H. poli. H. sanctori also showed a significant toxic effect against PANC-1, MDA-MB-231, HepG2 and A549 cells, whereas the IC50 value in CCD-34LU cells was above 50 mu g/mL for this sample. The n-butanol extracts of sea cucumber species reduced hemagglutination (HA) virus titer between 1-fold to 4-fold in log2-based at all tested concentrations. The best inhibited virus HA titer results were found in H. tubulosa at 5 mu g/g. According to these results we have obtained, the extracts of sea cucumbers may be used in many fields such as medicine, food, cosmetics in the future. This study is also very important in terms of being a guide for all studies on the use, processing and production of sea cucumbers and detailed isolation and purification studies on sea cucumber species from Turkey.

ASSESSING CULTURAL ECOSYSTEM SERVICES DURING THE COVID-19 PANDEMIC AT THE GARDEN OF NINFA (ITALY)

Green areas provide Cultural Ecosystem Services (CESs), that is, the ecosystem outputs that enable a range of experiential and intellectual activities. These include health promotion, recreation, enjoyment of the cultural heritage, and aesthetic experiences. The demand for CESs has grown during the first half of 2020, when most of the EU Member States had to face a stringent lockdown to contain the spread of the Covid-19, and people have undergone considerable psychophysical distress. In this framework, the Garden of Ninfa, one of the most visited Gardens of Italy, with its natural, historical, and architectural beauties delivers precious CESs, which have however been poorly studied. In this research, through a survey, we investigated the CESs delivered by the Garden of Ninfa in the immediate post-lockdown period, providing at the same time a monetary evaluation. The results show that people mostly visit the Garden of Ninfa for the aesthetic experience, followed by the resonance in terms of culture or heritage and health promotion;the combination of water bodies and fauna is highly effective in delivering CESs. The monetary evaluation, ranging between 1.0 and 2.7 Million EUR per year, may stimulate the replication of similar initiatives, especially in highly altered areas. The safety measures put in place by the governance of the Garden of Ninfa have safeguarded the tour experience from the Covid-19 fear of infection.

Traditional plants utilized for the viral disease treatment

Ethnobotanical research is a well-established field of science that attracts a lot of interest in medicine. Plants are responsible for over 80% of folk remedies used in primary care worldwide. Traditional and herbal medicine knowledge is essential in scientific research, especially when the literature and survey data are not adequately examined. Viral diseases affect millions of individuals worldwide, and they have a significant impact on human health and socioeconomic growth. Many infectious and non-infectious illnesses have long been treated with medicinal plants. The value of medicinal plants has risen in recent centuries. The human immunodeficiency virus (HIV) alone affects almost 40 million people. Coronavirus disease is now the most common viral illness globally, affecting an estimated 176 million people worldwide (COVID-19). A wide range of plant species was found to be effective in treating viral diseases. This review summarizes viral illness, disease outbreaks, and medicinal plants and herbs with antiviral properties useful in drug development programmes.

Potential roles of phytochemicals in combating severe acute respiratory syndrome Coronavirus infection

Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2), the causative agent of the current ongoing global pandemic COVID-19 is yet far away from the clutches of contemporary western medicines. With the lack of conventional drugs for this deadly disease the scope for the development of herbal formulations and Ayurvedic medication is finding a sound basis in the current scenario. The past two years has witnessed detailed and focused investigations on the biologically active constituents derived from a range of medicinal plants and their potential antiviral properties against SARSCoV-2. The promising results of these investigations have intrigued the medical and plant experts in pharmacognosy enough to consider herbal medicines and plant-based products as they are more effective in combating the COVID-19 crisis. However, a large-scale application of the same would require more focused and thorough research on this matter. This review is an attempt to describe the current and future prospects of using medicinal plants and herbal compounds as natural and sustainable alternative for treating COVID-19. The current article evaluates the various strong evidences from biochemical and molecular studies that have been investigated so far for the development of herbal formulations to combat COVID-19 with detailed focus on the most potential phytochemicals of medicinal plants studied in this regard namely, Withania somnifera (L.) Dunal, Cinchona officinalis L., Curcuma longa L., Ocimum sanctum L., Azadirachta indica A. Juss. and Tinospora cordifolia (Willd.) Miers.

Mudancas no processo de compra e consumo de alimentos organicos durante a pandemia do COVID-19

Inserted in an increasing production and consumption of organic food context, the world faced to a great adversity, the COVID-19 pandemic, causing a great impact on the lives of people worldwide, also including shopping and consumption process. Thus, the study aimed to identify the changes that occurred in the process of purchase and consumption of organic foods consumers, as well as the changes that occurred in the influences of this process during the COVID-19 pandemic. A qualitative research was conducted in which twelve consumers of organic products were interviewed through online interviews. The main results found in the study were changes regarding to situational influences due to the situation of the COVID-19 pandemic. The study also shows that regarding to the stages of the purchasing decision-making process, it was identified that changes occurred in all stages, and the stage in which the most changed occurred was the purchase and consumption stage.

Investigation of SARS-CoV-2 Main Protease Potential Inhibitory Activities of Some Natural Antiviral Compounds Via Molecular Docking and Dynamics Approaches

Coronaviruses caused an outbreak pandemic disease characterized by a severe acute respiratory distress syndrome leading to the infection of more than 200 million patients and the death of more than 4 million individuals. The primary treatment is either supportive or symptomatic. Natural products have an important role in the development of various drugs. Thus, screening of natural compounds with reported antiviral activities can lead to the discovery of potential inhibitory entities against coronaviruses. In the current study, an in-silico molecular docking experiment was conducted on the effects of some of these natural antiviral phytoconstituents, (e.g., procyanidin B2, theaflavin, quercetin, ellagic acid, caffeoylquinic acid derivatives, berginin, eudesm-1 beta, 6 alpha, 11-triol and arbutin), on the crystal structure of SARS-CoV-2 main protease (PDB ID: 6w63) using AutoDock-Vina software. Many of the docked compounds revealed good binding affinity, with procyanidin B2 (-8.6 Kcal/mol) and theaflavin (-8.5 Kcal/mol) showing a better or similar binding score as the ligand (-8.5 Kcal/mol). Molecular dynamics simulations were carried out at 100 ns and revealed that procyanidin B2 forms a more stable complex with SARS-CoV-2 main protease than theaflavin. Procyanidin B2, theaflavin, and 4,5-dicaffeoylquinic acid were evaluated for toxicity by ProTox-II webserver and were non-toxic according to the predicted LD50 values and safe on different organs and pathways. Additionally, these phytoconstituents showed good ADME properties and acceptable lipophilicity, as evaluated using WLOGP. Amongst the tested compounds, procyanidin B2 showed the highest lipophilic value. It is worth mentioning that these natural inhibitiors of SARS-CoV-2 main protease are components of green and black tea that can be used as a supporting supplement for COVID patients or as potential nuclei for further drug design and development campaigns.

Cloning and functional characterization of a terpene synthase gene AlTPS1 from Atractylodes lancea

Atractylodes lancea (Thunb.) DC has been used widely as a medicinal herb for centuries and is now being used to treat COVID-19 pneumonia. Terpenoids are thought to be its main pharmacologically active constituents. However, their biosynthesis remains uncharacterized in this species. In this study, the terpene synthase gene AlTPS1 was cloned and functionally characterized. We found that AlTPS1 was a bifunctional enzyme that catalyzed the conversion of farnesyl diphosphate to nerolidol and geranyl diphosphate to linalool in vitro. However, it functioned only in the nerolidol production in vivo by transient expression of the AlTPS1 gene in Nicotiana benthamiana leaves maybe due to subcellular compartmentalization of the AlTPS1 in the cytosol. Furthermore, AlTPS1 was highly expressed in leaves, considered to be the sites of nerolidol synthesis. This study is the first in which the cloning and expression of the AlTPS1 gene from A. lancea were analyzed, and it has provided new insights into terpene biosynthesis in A. lancea.